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Cas-9 expressing stocks available in the Fly Facility

The Cas-9 expressing stocks currently available in the Fly Facility are listed below. if the stock you want to inject is not on this list, please contact us at as we may have received it recently (CRISPR is a fast moving field!). If we do not keep the Cas-9 stock you need, please send some healthy flies for us to inject. Since we will need time to expand the stock, we recommend that you plan ahead and send the stock before you send your samples.

 

Cas-9 on X chromosome

 

  • CFD1 (Bloomington: 54590): act5C-cas9

Genotype:        y1 P(act5c-cas9, w+) M(3xP3-RFP.attP)ZH-2A w*

Expresses Cas9 protein ubiquitously under control of Act5C regulatory sequences. The attP landing site is marked with RFP.

 

  • CFD2 (Bloomington: 54591): nos-cas9

Genotype:        y1 P(nos-cas9, w+) M(3xP3-RFP.attP)ZH-2A w*

Expresses Cas9 protein in the germline under control of nanos regulatory sequences. The attP landing site is marked with RFP.

 

  • CFD3_nos (Bloomington: 54593): nanosGal4VP16 UAS-cas9

Genotype:        y1 P(UAS-cas9, w+) M(3xP3-RFP.attP)ZH-2A w*, nanosGal4VP16

Expresses Cas9 protein under UAS control. Expresses GAL4 in the germline under control of nanos regulatory sequences. The attP landing site is marked with RFP.

 

  • vas-cas9 (Bloomington: 51323): vas-cas9

Genotype:        y[1] M{vas-Cas9}ZH-2A w[1118]/FM7c

Expresses Cas9 protein in the germline under control of vasa regulatory sequences. The Cas9 insertion is marked with GFP and the attP landing site is marked with RFP.


  • vas-cas9 (Bloomington 55821):

Genotype:       y1 M{vas-Cas9.RFP-}ZH-2A w1118/FM7a, P{Tb1}FM7-A

Expresses Cas9 protein in the germline under control of vasa regulatory sequences. The Cas9 insertion is marked with GFP. The RFP marking the attP landing site has been removed.

 

Cas-9 on 2nd chromosome

 

  • TH_attP40

y[1] sc[1] v[1]; {y[+t7.7] v[+t1.8]=nanos-Cas9}attp40

Expresses Cas9 protein in the germline under control of nanos regulatory sequences. The Cas9 insert on the 2nd chromosome makes this stock useful when targeting X chromosome sequences. 

 

Cas-9 on 3rd chromosome

 

  • TH_attP2

y[1] sc[1] v[1];; {y[+t7.7] v[+t1.8]=nanos-Cas9}attp2

Expresses Cas9 protein in the germline under control of nanos regulatory sequences. The Cas9 insert on the 3rd chromosome makes this stock useful when targeting X chromosome sequences.

Contact Information

E-mail contacts

Simon Collier (Fly Facility Manager): s.collier@gen.cam.ac.uk

General Fly Facility enquiries: flyadmin@gen.cam.ac.uk

Microinjection enquiries: flyinject@gen.cam.ac.uk

Fly food enquiries: flymedia@gen.cam.ac.uk

Fly stock requests: gh19@gen.cam.ac.uk

Address

Department of Genetics,
University of Cambridge, Downing Street,
Cambridge CB2 3EH,
United Kingdom

Phone: +44 (0)1223 765124
Fax: +44 (0)1223 333992

 

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