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Drosophila Microinjection - All you need to know

Important: Preparing your DNA sample

DNA quality and quantity for embryo injection - The embryo survival rate directly depends on the purity of the plasmid DNA, therefore DNA for embryo injection needs to be of Qiagen midiprep quality, or equivalent. When using the Qiagen miniprep kit, you should include the 'optional' PB buffer wash step. The ratio of OD260/280 should be 1.80-1.90. Please be aware that significant delays can result if your sample purity does not meet these criteria. The concentration of the plasmid for injection should be 1μg/μl (0.4μg/μl for phiC31 injections) dissolved in dd (distilled,deionized) sterile water (not T.E.), and a minimum of 10μl is required. We provide the injection buffer and the P-element helper plasmid (if required). 

 

Help with experimental design

We are happy to assist with selecting the best host stock (embryos) for your microinjection experiment and with other aspects of experimental design. Please contact us at flyinject@gen.cam.ac.uk. If you prefer a host stock that is not currently kept by the Fly Facility, please send us some healthy flies or direct us to where we can obtain some.

 

Sending your samples for microinjection

Please download and complete the Request for Drosophila Microinjection Work form and send it with your samples for injection to Kadri Oras, Department of Genetics, Downing Site, Downing Street, Cambridge, CB2 3EH. UK.

 

Paying for your microinjection work

Click here for a description of our prices and plans

  • Users from outside the University of Cambridge Department of Genetics: Please email copies of a Purchase Order to (simon.collier@gen.cam.ac.uk) at the time you send your samples. Invoices for microinjection work are normally sent at the completion of the service unless you request otherwise.

 

VAT charges for Microinjection work (March 2017)

We have recently been informed by the University of Cambridge Finance Division Tax Office that our Microinjection Service does not qualify for medical VAT-exmption. Consequently all future microinjection charges for UK research groups (outside of the University of Cambridge) will be subject to VAT at the standard rate (20%).

 

What if my microinjection experiment fails? 

For Plans 2, 3, 5 and 7 we will inject a further 200 embryos (at no extra cost) if we do not obtain our guaranteed number of transformants. However, Plans 1, 4, 6 and 8 are injection only – we do not routinely re-inject if no transformants are obtained. Click here for a description of Plans 1-8.

 

Acknowledging the Fly Facility

Please acknowledge 'The University of Cambridge Department of Genetics Fly Facility' in any publications or public presentations resulting from the use of microinjected flies

 

Our Terms and Conditions

To use our microinjection service, you must agree to our Term and Conditions for Microinjection work.

Contact Information

E-mail contacts

Simon Collier (Fly Facility Manager): s.collier@gen.cam.ac.uk

General Fly Facility enquiries: flyadmin@gen.cam.ac.uk

Microinjection enquiries: flyinject@gen.cam.ac.uk

Fly food enquiries: flymedia@gen.cam.ac.uk

Fly stock requests: gh19@gen.cam.ac.uk

Address

Department of Genetics,
University of Cambridge, Downing Street,
Cambridge CB2 3EH,
United Kingdom

Phone: +44 (0)1223 765124
Fax: +44 (0)1223 333992

 

The Fly Facility webpages are maintained by Simon Collier. Please contact me ) if you have any problems using the site.