skip to primary navigationskip to content
 

Drosophila Microinjection - All you need to know

Important: Preparing your DNA sample

Sample quality is the major factor that determines the success or failure of microinjection work. Please read our guidelines for sample preparation before sending your samples for microinjection.

 

Help with experimental design

We are happy to assist with selecting the best host stock (embryos) for your microinjection experiment and with other aspects of experimental design. Please contact us at flyinject@gen.cam.ac.uk. If you prefer a host stock that is not currently kept by the Fly Facility, please send us some healthy flies or direct us to where we can obtain some.

 

Sending your samples for microinjection

Please download and complete the Request for Drosophila Microinjection Work form and send it with your samples for injection to Alla Madich, Department of Genetics, Downing Site, Downing Street, Cambridge, CB2 3EH. UK.

If your sample tube is crushed during transport, your sample may be lost through the lid opening or the tube splitting. Therefore, it is best to mail your sample in a padded envelope (Jiffy bag) or a box. Sealing the lid with parafilm or similar will help prevent the lid opening and also guard against evaporation.

 

Sending fly stocks for microinjection

If you would like us to inject into your own fly line, or into the progeny of a cross that you have set up, please provide us with sufficient flies to make an injection cage. To set up a cage, we need a minimum of 500 flies (300 females, 200 males) and would prefer closer to 1000 flies. Please make sure the flies you provide are free of mites or we may not accept them for injections.

 

Receiving your injected flies

When the service is complete, we will send the injected flies to you via regular mail. In our experience, this approach works well and it is rare that the flies fail to reach their destination. For service plans that involve screening, crossing and/or stock construction (e.g. Plans 2, 3, 5 and 7) we will resend flies at no additional cost if the original package fails to reach you. However, for 'injection only' requests where we ship all the injected larvae (e.g. Plans 1, 4, 6 and 8) we will not repeat the service if you fail to receive the larvae, unless the failure clearly results from our own error. Therefore, for 'injection only' requests, we also give you the option of arranging alternative shipping (e.g. courier service) at your own expense. In this case, you take responsibility for ensuring that all the necessary information is proivided to the Fly Facility to ensure a successful shipment.

 

Paying for your microinjection work

Click here for a description of our prices and plans

  • University of Cambridge users outside of the Department of Genetics: Please email a copy of your Purchase Order to (simon.collier@gen.cam.ac.uk) at the time you send your samples. Also, please include the Purchase Order number on your Request for Drosophila Microinjection Work form. Invoices for microinjection work are normally sent at the completion of the service unless you request otherwise
  • Users outside of the University of Cambridge: You have two options:
  • Option 1: Purchase Order: Please email a copy of your Purchase Order to (simon.collier@gen.cam.ac.uk) at the time you send your samples. Also, please include the Purchase Order number on your Request for Drosophila Microinjection Work form. Invoices for microinjection work are normally sent at the completion of the service unless you request otherwise
  • Option 2: Credit card:
  1. Credit card users in the UK: Please visit our Online Store for UK Business. Enter the total cost of the sevice including VAT (all UK users outside of the University of Cambridge are required to pay VAT at 20% on their microinjection payments). You will be asked to provide the name of the person who submitted the original Microinjection Request Form as well as their department and institution affiliation. Please provide this information, it will allow us to confidently match your payment to a specific microinjection request.
  2. Credit card users in the rest of the European Community: Please visit our Online Store for EC Business. Enter the total cost of the service excluding VAT. You will be asked to provide the name of the person who submitted the original Microinjection Request Form as well as their department and institution affiliation. Please provide this information, it will allow us to confidently match your payment to a specific microinjection request.

 

VAT charges for Microinjection work

We have been informed by the University of Cambridge Finance Division Tax Office that our Microinjection Service does not qualify for medical VAT-exmption. Consequently all microinjection charges for UK research groups (outside of the University of Cambridge) are subject to VAT at the standard rate (20%).

 

What if my microinjection experiment fails? 

For Plans 2, 3, 5 and 7 we will inject a further 200 embryos (at no extra cost) if we do not obtain our guaranteed number of transformants. However, Plans 1, 4, 6 and 8 are injection only – we do not routinely re-inject if no transformants are obtained. Click here for a description of Plans 1-8.

 

Acknowledging the Fly Facility

Please acknowledge 'The University of Cambridge Department of Genetics Fly Facility' in any publications or public presentations resulting from the use of microinjected flies

 

Our Terms and Conditions

  1. To use our microinjection service, you must agree to our Term and Conditions for Microinjection work.

Contact Information

Tiny fly

E-mail contacts

Simon Collier (Fly Facility Manager): s.collier@gen.cam.ac.uk

General Fly Facility enquiries: flyadmin@gen.cam.ac.uk

Microinjection enquiries: flyinject@gen.cam.ac.uk

Fly food enquiries: flymedia@gen.cam.ac.uk

Fly stock requests: gh19@gen.cam.ac.uk

Address

Department of Genetics,
University of Cambridge, Downing Street,
Cambridge CB2 3EH,
United Kingdom

Phone: +44 (0)1223 765124
Fax: +44 (0)1223 333992

 

The Fly Facility webpages are maintained by Simon Collier. Please contact me ) if you have any problems using the site.