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Fly Pushing Projects

flp eye

Our Fly Pushing Services

Our fly pushing services are primarily aimed at UK fly researchers, but we are happy to consider requests from further afield. We can provide support for a range of Drosophila work including crosses, stock-construction, screens and also larger (genome-wide) projects. Charges for our Fly Pushing service are arrived at by negotiation between the research group and the Fly Facility. Examples of our work with the Jefferis group (MRC-LMB, Cambridge) and Reddy group (IMS, Cambridge) are outlined below.

 

Recent Fly Facility project work

Our work with the Jefferis group (MRC-LMB, Cambridge)

Jefferis Brain 2

The Jefferis group (MRC-LMB, Cambridge) studies circuit basis of innate behaviour in the fly. Approaches include anatomical work to characterise the structure and connectivity of relevant neurons in the brain, electrophysiology to monitor their activity and molecular genetic studies of behaviour that perturb the function or development of the identified neurons. All of this work depends on the ability to target specific neuronal populations of interest. Glynnis Johnson, Senior Research Technician in the Fly Facility, carried out a screen for the Jefferis group to generate new split gal4 enhancer trap lines that could be used to label highly specific neuronal populations. The Fly Facility worked with the Jefferis group to design the screen which involved mobilizing a P-element enhancer trap to generate new insertions (and expression patterns) and crossing these to reporter flies. Brains from the progeny were then screened anatomically by the Jefferis group. Selected lines were balanced (mapped to a chromosome) by Glynnis, and some lines were then recombined with transgenes from the reporter flies. Finally the enhancer trap lines for which behavioural experiments are planned were outcrossed.

 

Our work with the Reddy group (IMS, Cambridge)

The Reddy group (IMS, Cambridge) studies circadian rhythms and sleep. The group is especially interested in redox and metabolic oscillations within cells and tissues that appear to be a core feature of circadian clocks. The Fly Facility has worked with the Reddy group to generate knockdown flies with reduced expression of various redox genes. Preliminary data from these flies suggest that knockdown of specific redox genes in the fly brain can affect sleep and/or circadian behaviours.

Utham figure

Contact Information

Tiny fly

E-mail contacts

Simon Collier (Fly Facility Manager): s.collier@gen.cam.ac.uk

General Fly Facility enquiries: flyadmin@gen.cam.ac.uk

Microinjection enquiries: flyinject@gen.cam.ac.uk

Fly food enquiries: flymedia@gen.cam.ac.uk

Fly stock requests: gh19@gen.cam.ac.uk

Address

Department of Genetics,
University of Cambridge, Downing Street,
Cambridge CB2 3EH,
United Kingdom

Phone: +44 (0)1223 765124
Fax: +44 (0)1223 333992

 

The Fly Facility webpages are maintained by Simon Collier. Please contact me ) if you have any problems using the site.