phiC31 stocks available in the Fly Facility

The phiC31 expressing stocks currently available in the Fly Facility are listed below. If the phiC31 stock you want to inject is not on our list, please contact us at flyinject@gen.cam.ac.uk as we may have received it recently. If we do not keep the  stock you need, please send some healthy flies for us to inject. Our guidelines to sending stocks for microinjection workare in section 4 of our 'Drosophila Microinjection - All you need to know' page.

X Chromosome attP Landing Site  

attP18 (Bloomington Stock 32107)

Genotype: y[1] w[67c23] P{y[+t7.7]=CaryP}attP18

attP site: X Chromosome (6C12) marked with yellow+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

attP3 (BloomingtonStock 32230)

Genotype: y[1] w[*] P{y[+t7.7]=CaryP}attP3

attP site: X Chromosome (19C4) marked with yellow+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

attP(2Ar5) (BloomingtonStock 24480)

Genotype: y[1] M{3xP3-RFP.attP}ZH-2A w[*]; M{vas-int.Dm}ZH-102D

attP site: X Chromosome (2A3) marked with RFP eye expression

phiC31 integrase: Chromosome 4 (102F4), vasa promoter, marked with GFP and RFP

Chromosome 2 attP Landing Site  

vas-int; attP40 (Stock 13-20)

Genotype: y w M(eGFP, vas-int, dmRFP)ZH-2A; P{CaryP}attP40

attP site: 2nd chromosome (25C6), marked with yellow+

phiC31 integrase: X chromosome (2A3), vasa promoter, marked with GFP and RFP

y, v, nos-int; attP40 (Bloomington stock 25709)

Genotype: y[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X; P{y[+t7.7]=CaryP}attP40

attP site: 2nd chromosome (25C6), marked with yellow+

phiC31 integrase: X chromosome (2A3), nanos promoter, marked with yellow+

Please note: This stock is a vermillion mutant and so is not an appropriate line for constructs carrying white+

vas-Int; attp51C (Bloomington stock 24482)

Genotype:y[1] M{vas-int.Dm}ZH-2A w*; M{3xP3-RFP.attP'}ZH-51C

attP site: 2nd chromosome (51C), marked with RFP

phiC31 integrase: X chromosome, vasa promoter

vas-int; attp-51D  (Stock 12-100)

Genotype: y w M(eGFP, vas-int, dmRFP) ZH-2A; M(attP)ZH-51D

attP site: 2nd chromosome (51D) – unmarked

phiC31 integrase: X chromosome, vasa promoter, marked with GFP and RFP

Note: Bloomington stock 24483 with attP RFP marker removed (cre-ed out) – may be problems with enhancer trap effects at 51D

attP1 (Bloomington Stock8621)

Genotype: y[1] w[67c23]; P{y[+t7.7]=CaryP}attP1

attP site: 2nd chromosome (55C4) – marked with yellow+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

Chromosome 3 attP Landing Site

nos-int; attP2 (Stock 13-18)

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; P{y[+t7.7]=CaryP}attP2

attP site: 3rd chromosome (68A4), marked with yellow+

phiC31 integrase: X chromosome, nanos promoter, marked with yellow+

y, v, nos-int; attP2 (Bloomington Stock 25710)

Genotype: y[1] sc[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X;; P{y[+t7.7]=CaryP}attP2

attP site: 3rd chromosome (68A4), marked with yellow+

phiC31 integrase: X chromosome, nanos promoter, marked with yellow+

Please note: This stock is a vermillion mutant and so is not an appropriate line for constructs carrying white+

vas-int; attP-86Fb(Bloomington Stock24749 )

Genotype:y[1] M{RFP[3xP3.PB] GFP[E.3xP3]=vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP}ZH-86Fb

attP site: 3rd chromosome (86F8) – marked with 3xP3-RFP

phiC31 integrase: X chromosome, vasa promoter, marked with GFP and RFP

VK00005 (Stock 13-22)

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00005

attP site: 3rd chromosome (75A10), marked with yellow+

phiC31 integrase: X chromosome, nanos promoter, marked with yellow+

VK00013 RFP-, GFP-

Genotype: y[1] w* M{vasint.Dm}ZH2A;; PBac{y[+]-attP-9A]VK00013

attP site: 3rd chromosome (76A2), marked with yellow+

phiC31 integrase: X chromosome (2A3), vasa promoter

Note: RFP and GFP markers removed from the integrase insertion site

VK00033(Stock 13-106)  

Genotype: y w M(eGFP, vas-int, dmRFP)ZH-2A;; PBac{y[+]-attP-3B}VK00033

attP site: 3rd chromosome, marked with yellow+

phiC31 integrase: X chromosome (2A3), vasa promoter, marked with GFP and RFP

VK00040 (Stock 13-24)

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00040

attP site: 3rd chromosome (87B10), marked with yellow+

phiC31 integrase: X chromosome, nanos promoter, marked with yellow+

Balancer chromosomes (X, 2nd and 3rd) with attP Landing Sites

Bloomington Stock 38451: w;; Dr/TM3 attp(FS10) Sb Ser

Genotype:  w[1118]; Dr[1]/TM3, P{w[+mC]=attP.w[+].attP}FS10 Sb[1] Ser[1]

attP site: On TM3 3rd chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

Bloomington Stock 38452: w;; Dr/TM3 attp(FS18b) Sb Ser

Genotype:   w[1118]; Dr[1]/TM3, Sb[1] Ser[1] P{w[+mC]=attP.w[+].attP}FS18

attP site: On TM3 3rd chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

Bloomington Stock 38453: yw; Sco/CyO, attp(J04)

Genotype:   y[1] w[*]; sna[Sco]/CyO, P{w[+mC]=attP.w[+].attP}J04

attP site: On CyO 2nd chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

Bloomington Stock 38454: yw; Sco/CyO, attp(J08)

Genotype:   y[1] w[*]; sna[Sco]/CyO, P{w[+mC]=attP.w[+].attP}J08

attP site: On CyO 2nd chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

Bloomington Stock 38455: FM7h, attp(FS2)/C(1)DX

Genotype:   FM7h, P{w[+mC]=attP.w[+].attP}FS2/C(1)DX, y[1] f[1]

attP site: On FM7h X chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

Bloomington Stock 38456: FM7h, attp(FS5)/C(1)DX

Genotype:   FM7h, P{w[+mC]=attP.w[+].attP}FS5/C(1)DX, y[1] f[1]

attP site: On FM7h X chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required