phiC31 stocks available in the Fly Facility

The phiC31 expressing stocks currently available in the Fly Facility are listed below. if the stock you want to inject is not on this list, please contact us at flyinject@gen.cam.ac.uk as we may have received it recently. If we do not keep the phiC31 stock you need, please send some healthy flies for us to inject. Since we will need time to expand the stock, we recommend that you plan ahead and send the stock before you send your samples.

X Chromosome attP Landing Site  

Bloomington Stock 32107

Genotype: y[1] w[67c23] P{y[+t7.7]=CaryP}attP18

attP site: X Chromosome (6C12) marked with yellow+

Bloomington Stock 32230

Genotype: y[1] w[*] P{y[+t7.7]=CaryP}attP3

attP site: X Chromosome (19C4) marked with yellow+

Chromosome 2 attP Landing Site  

Stock 13-20: vas-int; attp40

Genotype: y w M(eGFP, vas-int, dmRFP)ZH-2A; P{CaryP}attP40

attP site: 2^nd chromosome (25C6), marked with yellow⁺

phiC31 integrase: X chromosome (2A3), vasa promoter, marked with GFP and RFP

Bloomington stock 25709: y, v, nos-int; attp40

Genotype: y[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X; P{y[+t7.7]=CaryP}attP40

attP site: 2^nd chromosome (25C6), marked with yellow⁺

phiC31 integrase: X chromosome (2A3), nanos promoter, marked with yellow+

Stock 12-100: vas-int; attp-51D    

Genotype: y w M(eGFP, vas-int, dmRFP) ZH-2A; M(attP)ZH-51D

attP site: 2^nd chromosome (51D) – unmarked

phiC31 integrase: X chromosome, vasa promoter, marked with GFP and RFP

Note: Bloomington stock 24483 with attP RFP marker removed (cre-ed out) – may be problems with enhancer trap effects at 51D

Chromosome 3 attP Landing Site

Stock 13-18: nos-int; attp2

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; P{y[+t7.7]=CaryP}attP2

attP site: 3^rd chromosome (68A4), marked with yellow⁺

phiC31 integrase: X chromosome, nanos promoter, marked with yellow⁺

Bloomington Stock 25710: y, v, nos-int; attp2

Genotype: y[1] sc[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X;; P{y[+t7.7]=CaryP}attP2

attP site: 3^rd chromosome (68A4), marked with yellow⁺

phiC31 integrase: X chromosome, nanos promoter, marked with yellow⁺

Stock 13-14: vas-int; attp-86Fb          

Genotype:  y w M(eGFP, vas-int, dmRFP)ZH-2A;; M(attP)ZH-86Fb

attP site: 3^rd chromosome (86F8) – unmarked

phiC31 integrase: X chromosome, vasa promoter, marked with GFP and RFP

Note: Bloomington stock 24749 with attP RFP marker removed (cre-ed out)

Stock 13-106: vas-int; attp-3B VK00033   

Genotype: y w M(eGFP, vas-int, dmRFP)ZH-2A;; PBac{y[+]-attP-3B}VK00033

attP site: 3^rd chromosome, marked with yellow⁺

phiC31 integrase: X chromosome (2A3), vasa promoter, marked with GFP and RFP

Stock 13-22: VK00005

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00005

attP site: 3^rd chromosome (75A10), marked with yellow⁺

phiC31 integrase: X chromosome, nanos promoter, marked with yellow⁺

Stock 13-23: VK00027

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00027

attP site: 3^rd chromosome (89E11), marked with yellow⁺

phiC31 integrase: X chromosome, nanos promoter, marked with yellow⁺

Stock 13-24: VK00040

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00040

attP site: 3^rd chromosome (87B10), marked with yellow⁺

phiC31 integrase: X chromosome, nanos promoter, marked with yellow⁺

VK00013 (76A) RFP-, GFP-

Genotype: y[1] w* M{vasint.Dm}ZH2A;; PBac{y[+]-attP-9A]VK00013

attP site: 3^rd chromosome (76A2), marked with yellow⁺

phiC31 integrase: X chromosome (2A3), vasa promoter

Note: RFP and GFP markers removed from the integrase