The phiC31 expressing stocks currently available in the Fly Facility are listed below. if the stock you want to inject is not on this list, please contact us at flyinject@gen.cam.ac.uk as we may have received it recently. If we do not keep the phiC31 stock you need, please send some healthy flies for us to inject. Our guidelines to sending stocks for microinjection work are in section 4 of our 'Drosophila Microinjection - All you need to know' page.
X Chromosome attP Landing Site
attP18 (Bloomington Stock 32107)
Genotype: y[1] w[67c23] P{y[+t7.7]=CaryP}attP18
attP site: X Chromosome (6C12) marked with yellow+
Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required
attP3 (Bloomington Stock 32230)
Genotype: y[1] w[*] P{y[+t7.7]=CaryP}attP3
attP site: X Chromosome (19C4) marked with yellow+
Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required
attP(2Ar5) (Bloomington Stock 24480)
Genotype: y[1] M{3xP3-RFP.attP}ZH-2A w[*]; M{vas-int.Dm}ZH-102D
attP site: X Chromosome (2A3) marked with RFP eye expression
phiC31 integrase: Chromosome 4 (102F4), vasa promoter, marked with GFP and RFP
Chromosome 2 attP Landing Site
vas-int; attP40 (Stock 13-20)
Genotype: y w M(eGFP, vas-int, dmRFP)ZH-2A; P{CaryP}attP40
attP site: 2nd chromosome (25C6), marked with yellow+
phiC31 integrase: X chromosome (2A3), vasa promoter, marked with GFP and RFP
attP40 GFP- RFP- (From Matthias Soller)
Genotype: y[1] w* M{vasint.Dm}ZH2A;; attP40
attP site: 2nd chromosome (25C6), marked with yellow+
phiC31 integrase: X chromosome (2A3), vasa promoter, GFP and RFP markers have been removed (Cre'd out)
y, v, nos-int; attP40 (Bloomington stock 25709)
Genotype: y[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X; P{y[+t7.7]=CaryP}attP40
attP site: 2nd chromosome (25C6), marked with yellow+
phiC31 integrase: X chromosome (2A3), nanos promoter, marked with yellow+
vas-Int; attp51C (Stock KK0018)
Genotype: y w vas-int; (attP)51C
attP site: 2nd chromosome (51C)
phiC31 integrase: X chromosome, vasa promoter
vas-int; attp-51D (Stock 12-100)
Genotype: y w M(eGFP, vas-int, dmRFP) ZH-2A; M(attP)ZH-51D
attP site: 2nd chromosome (51D) – unmarked
phiC31 integrase: X chromosome, vasa promoter, marked with GFP and RFP
Note: Bloomington stock 24483 with attP RFP marker removed (cre-ed out) – may be problems with enhancer trap effects at 51D
attP1 (Bloomington Stock 8621)
Genotype: y[1] w[67c23]; P{y[+t7.7]=CaryP}attP1
attP site: 2nd chromosome (55C4) – marked with yellow+
Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required
Chromosome 3 attP Landing Site
nos-int; attP2 (Stock 13-18)
Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; P{y[+t7.7]=CaryP}attP2
attP site: 3rd chromosome (68A4), marked with yellow+
phiC31 integrase: X chromosome, nanos promoter, marked with yellow+
y, v, nos-int; attP2 (Bloomington Stock 25710)
Genotype: y[1] sc[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X;; P{y[+t7.7]=CaryP}attP2
attP site: 3rd chromosome (68A4), marked with yellow+
phiC31 integrase: X chromosome, nanos promoter, marked with yellow+
vas-int; attP-86Fb (Stock 13-14)
Genotype: y w M(eGFP, vas-int, dmRFP)ZH-2A;; M(attP)ZH-86Fb
attP site: 3rd chromosome (86F8) – unmarked
phiC31 integrase: X chromosome, vasa promoter, marked with GFP and RFP
Note: Bloomington stock 24749 with attP RFP marker removed (cre-ed out)
VK00005 (Stock 13-22, Bloomington Stock 24862)
Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00005
attP site: 3rd chromosome (75A10), marked with yellow+
phiC31 integrase: X chromosome, nanos promoter, marked with yellow+
VK00013 RFP-, GFP-
Genotype: y[1] w* M{vasint.Dm}ZH2A;; PBac{y[+]-attP-9A]VK00013
attP site: 3rd chromosome (76A2), marked with yellow+
phiC31 integrase: X chromosome (2A3), vasa promoter
Note: RFP and GFP markers removed from the integrase insertion site
VK00027 (Stock 13-23)
Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00027
attP site: 3rd chromosome (89E11), marked with yellow+
phiC31 integrase: X chromosome, nanos promoter, marked with yellow+
VK00033 (Stock 13-106)
Genotype: y w M(eGFP, vas-int, dmRFP)ZH-2A;; PBac{y[+]-attP-3B}VK00033
attP site: 3rd chromosome, marked with yellow+
phiC31 integrase: X chromosome (2A3), vasa promoter, marked with GFP and RFP
VK00040 (Stock 13-24)
Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00040
attP site: 3rd chromosome (87B10), marked with yellow+
phiC31 integrase: X chromosome, nanos promoter, marked with yellow+
Balancer chromosomes (X, 2nd and 3rd) with attP Landing Sites
Bloomington Stock 38451: w;; Dr/TM3 attp(FS10) Sb Ser
Genotype: w[1118]; Dr[1]/TM3, P{w[+mC]=attP.w[+].attP}FS10 Sb[1] Ser[1]
attP site: On TM3 3rd chromosome balancer marked with white+
Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required
Bloomington Stock 38452: w;; Dr/TM3 attp(FS18b) Sb Ser
Genotype: w[1118]; Dr[1]/TM3, Sb[1] Ser[1] P{w[+mC]=attP.w[+].attP}FS18
attP site: On TM3 3rd chromosome balancer marked with white+
Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required
Bloomington Stock 38453: yw; Sco/CyO, attp(J04)
Genotype: y[1] w[*]; sna[Sco]/CyO, P{w[+mC]=attP.w[+].attP}J04
attP site: On CyO 2nd chromosome balancer marked with white+
Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required
Bloomington Stock 38454: yw; Sco/CyO, attp(J08)
Genotype: y[1] w[*]; sna[Sco]/CyO, P{w[+mC]=attP.w[+].attP}J08
attP site: On CyO 2nd chromosome balancer marked with white+
Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required
Bloomington Stock 38455: FM7h, attp(FS2)/C(1)DX
Genotype: FM7h, P{w[+mC]=attP.w[+].attP}FS2/C(1)DX, y[1] f[1]
attP site: On FM7h X chromosome balancer marked with white+
Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required
Bloomington Stock 38456: FM7h, attp(FS5)/C(1)DX
Genotype: FM7h, P{w[+mC]=attP.w[+].attP}FS5/C(1)DX, y[1] f[1]
attP site: On FM7h X chromosome balancer marked with white+
Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required
