skip to primary navigationskip to content
 

phiC31 stocks available in the Fly Facility

The phiC31 expressing stocks currently available in the Fly Facility are listed below. if the stock you want to inject is not on this list, please contact us at as we may have received it recently. If we do not keep the phiC31 stock you need, please send some healthy flies for us to inject. Our guidelines to sending stocks for microinjection work are in section 4 of our 'Drosophila Microinjection - All you need to know' page.

 

X Chromosome attP Landing Site  

 

attP18 (Bloomington Stock 32107)

Genotype: y[1] w[67c23] P{y[+t7.7]=CaryP}attP18

attP site: X Chromosome (6C12) marked with yellow+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

 

attP3 (Bloomington Stock 32230)

Genotype: y[1] w[*] P{y[+t7.7]=CaryP}attP3

attP site: X Chromosome (19C4) marked with yellow+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

 

attP(2Ar5) (Bloomington Stock 24480)

Genotype: y[1] M{3xP3-RFP.attP}ZH-2A w[*]; M{vas-int.Dm}ZH-102D

attP site: X Chromosome (2A3) marked with RFP eye expression

phiC31 integrase: Chromosome 4 (102F4), vasa promoter, marked with GFP and RFP


 

Chromosome 2 attP Landing Site  

 

vas-int; attP40 (Stock 13-20)

Genotype: y w M(eGFP, vas-int, dmRFP)ZH-2A; P{CaryP}attP40

attP site: 2nd chromosome (25C6), marked with yellow+

phiC31 integrase: X chromosome (2A3), vasa promoter, marked with GFP and RFP

 

attP40 GFP- RFP- (From Matthias Soller)

Genotype:  y[1] w* M{vasint.Dm}ZH2A;; attP40

attP site: 2nd chromosome (25C6), marked with yellow+

phiC31 integrase: X chromosome (2A3), vasa promoter, GFP and RFP markers have been removed (Cre'd out)

 

y, v, nos-int; attP40 (Bloomington stock 25709)

Genotype: y[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X; P{y[+t7.7]=CaryP}attP40

attP site: 2nd chromosome (25C6), marked with yellow+

phiC31 integrase: X chromosome (2A3), nanos promoter, marked with yellow+

 

vas-Int; attp51C (Stock KK0018)

Genotype: y w vas-int; (attP)51C

attP site: 2nd chromosome (51C)

phiC31 integrase: X chromosome, vasa promoter

 

vas-int; attp-51D  (Stock 12-100)

Genotype: y w M(eGFP, vas-int, dmRFP) ZH-2A; M(attP)ZH-51D

attP site: 2nd chromosome (51D) – unmarked

phiC31 integrase: X chromosome, vasa promoter, marked with GFP and RFP

Note: Bloomington stock 24483 with attP RFP marker removed (cre-ed out) – may be problems with enhancer trap effects at 51D

 

attP1 (Bloomington Stock 8621)

Genotype: y[1] w[67c23]; P{y[+t7.7]=CaryP}attP1

attP site: 2nd chromosome (55C4) – marked with yellow+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required


 

Chromosome 3 attP Landing Site

 

nos-int; attP2 (Stock 13-18)

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; P{y[+t7.7]=CaryP}attP2

attP site: 3rd chromosome (68A4), marked with yellow+

phiC31 integrase: X chromosome, nanos promoter, marked with yellow+

 

y, v, nos-int; attP2 (Bloomington Stock 25710)

Genotype: y[1] sc[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X;; P{y[+t7.7]=CaryP}attP2

attP site: 3rd chromosome (68A4), marked with yellow+

phiC31 integrase: X chromosome, nanos promoter, marked with yellow+

 

vas-int; attP-86Fb (Stock 13-14)      

Genotype:  y w M(eGFP, vas-int, dmRFP)ZH-2A;; M(attP)ZH-86Fb

attP site: 3rd chromosome (86F8) – unmarked

phiC31 integrase: X chromosome, vasa promoter, marked with GFP and RFP

Note: Bloomington stock 24749 with attP RFP marker removed (cre-ed out)

 

VK00005 (Stock 13-22)

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00005

attP site: 3rd chromosome (75A10), marked with yellow+

phiC31 integrase: X chromosome, nanos promoter, marked with yellow+

 

VK00013 RFP-, GFP-

Genotype: y[1] w* M{vasint.Dm}ZH2A;; PBac{y[+]-attP-9A]VK00013

attP site: 3rd chromosome (76A2), marked with yellow+

phiC31 integrase: X chromosome (2A3), vasa promoter

Note: RFP and GFP markers removed from the integrase insertion site

 

VK00027 (Stock 13-23)

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00027

attP site: 3rd chromosome (89E11), marked with yellow+

phiC31 integrase: X chromosome, nanos promoter, marked with yellow+

 

VK00033 (Stock 13-106)  

Genotype: y w M(eGFP, vas-int, dmRFP)ZH-2A;; PBac{y[+]-attP-3B}VK00033

attP site: 3rd chromosome, marked with yellow+

phiC31 integrase: X chromosome (2A3), vasa promoter, marked with GFP and RFP

 

VK00040 (Stock 13-24)

Genotype: y w P{y[+t7.7]=nos-phiC31\int.NLS}X #12;; PBac{y+-attP-9A}VK00040

attP site: 3rd chromosome (87B10), marked with yellow+

phiC31 integrase: X chromosome, nanos promoter, marked with yellow+

 


 

Balancer chromosomes (X, 2nd and 3rd) with attP Landing Sites

 

Bloomington Stock 38451: w;; Dr/TM3 attp(FS10) Sb Ser

Genotype:  w[1118]; Dr[1]/TM3, P{w[+mC]=attP.w[+].attP}FS10 Sb[1] Ser[1]

attP site: On TM3 3rd chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

 

Bloomington Stock 38452: w;; Dr/TM3 attp(FS18b) Sb Ser

Genotype:   w[1118]; Dr[1]/TM3, Sb[1] Ser[1] P{w[+mC]=attP.w[+].attP}FS18

attP site: On TM3 3rd chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

 

Bloomington Stock 38453yw; Sco/CyO, attp(J04)

Genotype:   y[1] w[*]; sna[Sco]/CyO, P{w[+mC]=attP.w[+].attP}J04

attP site: On CyO 2nd chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

 

Bloomington Stock 38454: yw; Sco/CyO, attp(J08)

Genotype:    y[1] w[*]; sna[Sco]/CyO, P{w[+mC]=attP.w[+].attP}J08

attP site: On CyO 2nd chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

 

Bloomington Stock 38455: FM7h, attp(FS2)/C(1)DX

Genotype:    FM7h, P{w[+mC]=attP.w[+].attP}FS2/C(1)DX, y[1] f[1]

attP site: On FM7h X chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

 

Bloomington Stock 38456: FM7h, attp(FS5)/C(1)DX

Genotype:    FM7h, P{w[+mC]=attP.w[+].attP}FS5/C(1)DX, y[1] f[1]

attP site: On FM7h X chromosome balancer marked with white+

Please note: This stock does not express PhiC31 integrase so co-injection of an integrase helper plasmid is normally required

Contact Information

Tiny fly

E-mail contacts

Simon Collier (Fly Facility Manager): s.collier@gen.cam.ac.uk

General Fly Facility enquiries: flyadmin@gen.cam.ac.uk

Microinjection enquiries: flyinject@gen.cam.ac.uk

Fly food enquiries: flymedia@gen.cam.ac.uk

Fly stock requests: gh19@gen.cam.ac.uk

Address

Department of Genetics,
University of Cambridge, Downing Street,
Cambridge CB2 3EH,
United Kingdom

Phone: +44 (0)1223 765124
Fax: +44 (0)1223 333992

 

The Fly Facility webpages are maintained by Simon Collier. Please contact me ) if you have any problems using the site.